The challenge for single parishioners is slightly different, though - to abstain from sex for 30 days, reports Sky News.
Paul Wirth, head pastor of the Relevant Church in Ybor City, said the marathon undertaking could help cut high divorce rates.
He said: "Couples across America are struggling in their relationships, both married and single people.
"For married people it seems like the sex is great up front, but then for some reason life happens."
But when you're single it's like you're always thinking about it and you're like, man I'd like to have it as much as possible.
"And sometimes that prevents them from having a great, healthy relationship later on when you do get married.
"The Relevant Church describes itself as "a casual, contemporary Christian church" and says its services are designed "specifically for urban professionals and young families".